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Anti-PDGFRA Polyclonal Antibody

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Catalog No. TMAB-01354

Anti-PDGFRA Polyclonal Antibody is a Rabbit antibody targeting PDGFRA. Anti-PDGFRA Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-PDGFRA Polyclonal Antibody

Anti-PDGFRA Polyclonal Antibody

😃Good
Catalog No. TMAB-01354
Anti-PDGFRA Polyclonal Antibody is a Rabbit antibody targeting PDGFRA. Anti-PDGFRA Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$373 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-PDGFRA Polyclonal Antibody is a Rabbit antibody targeting PDGFRA. Anti-PDGFRA Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse,Rat,Dog (predicted:Chicken,Pig,Cow,Horse)
Verified Activity
1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PDGFRA Polyclonal Antibody, Unconjugated (TMAB-01354) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PDGFRA Polyclonal Antibody, Unconjugated (TMAB-01354) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Blank control: Mouse Kidney (blue). Primary Antibody: Rabbit Anti-PDGFRA antibody (TMAB-01354,Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at 37°C. Primary antibody (TMAB-01354, 1 μg/1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min on ice.
4. Black line: Positive blank control (SH-SY5Y); Negative blank control (A431)
Green line: Primary Antibody (Rabbit Anti-PDGFRA antibody (TMAB-01354))
Orange line: Isotype Control Antibody (Rabbit IgG).
Blue line: Secondary Antibody (Goat anti-rabbit IgG-AF488)
SH-SY5Y (Positive) and A431 (Negative control) cells (black) were fixed with 4% PFA for 10 min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at-20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PDGFRA Antibody (TMAB-01354) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type I membrane protein. Note=The activated receptor is rapidly internalized and degraded.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PDGF-R-A
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
PDGF,Growth factor receptors,Growth Factors,Inflammatory mediators,PDGF,Receptor Tyrosine Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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