Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM

Reactivity: Human,Mouse,Rat (predicted:Pig,Cow,Horse)

IgG

Human,Mouse,Rat (predicted:Pig,Cow,Horse)

1. Blank control: 293T cells (blue). Primary Antibody: Rabbit Anti-PARK7/CAP1 antibody (TMAB-01332), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
Primary antibody (TMAB-01332, 0.2 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
2. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CAP1/PARK7 Polyclonal Antibody, Unconjugated (TMAB-01332) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CAP1/PARK7 Polyclonal Antibody, Unconjugated (TMAB-01332) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. The blue histogram is unstained cells (Hepg2 cells)
concentration 1:50
The Wathet Blue histogram is cells stained with secondary antibody alone.
The Orange histogram is cells stained with rabbit IgG isotype control antibody
plus secondary antibody.
The green histogram is cells stained with Rabbit Anti-PARK7/CAP1 antibody (TMAB-01332) plus secondary antibody.
5. Sample:
Lane 1: Testis (Mouse) Lysate at 40 μg
Lane 2: Liver (Mouse) Lysate at 40 μg
Lane 3: Cerebrum (Rat) Lysate at 40 μg
Lane 4: Thyroid gland Rat) Lysate at 40 μg
Lane 5: Kidney (Rat) Lysate at 40 μg
Lane 6: Liver (Rat) Lysate at 40 μg
Lane 7: Hela (Human) Cell Lysate at 30 μg
Lane 8: U937 (Human) Cell Lysate at 30 μg
Lane 9: K562 (Human) Cell Lysate at 30 μg
Lane 10: HL60 (Human) Cell Lysate at 30 μg
Primary:
Anti-PARK7/DJ1 (TMAB-01332) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
6. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PARK7) Polyclonal Antibody, Unconjugated (TMAB-01332) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PARK7) Polyclonal Antibody, Unconjugated (TMAB-01332) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CAP1

Human

11315

Small G proteins,DNA / RNA binding,Oxidative stress,Parkin / PARK,Regulators