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Anti-OPA1 Polyclonal Antibody
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Catalog No. TMAB-01288
Anti-OPA1 Polyclonal Antibody is a Rabbit antibody targeting OPA1. Anti-OPA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-OPA1 Polyclonal Antibody
Anti-OPA1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01288
Anti-OPA1 Polyclonal Antibody is a Rabbit antibody targeting OPA1. Anti-OPA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-OPA1 Polyclonal Antibody is a Rabbit antibody targeting OPA1. Anti-OPA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-OPA1 Polyclonal Antibody, Unconjugated (TMAB-01288) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Cerebrum (Rat) Lysate at 40 μg Primary: Anti-OPA1 (TMAB-01288) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kDa Observed band size: 111 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion inner membrane. Mitochondrion intermembrane space. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | OPA1 is a 120kDa protein belonging to the dynamin family. The OPA1 gene has been localized to 3q29. The gene is targeted to mitochondria and is involved in mitochondrial biogenesis. Defects in OPA1 are a cause of optic atrophy type 1. OPA1 is mostly expressed in retina but can also be expressed in brain, testis, heart and skeletal muscle. |
| Immunogen | KLH conjugated synthetic peptide: human OPA1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cardiac metabolism,Mitophagy fission and fusion,Neurodegenerative disease,Visual system |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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