Antibody Type: Rabbit Polyclonal

Application: WB,FCM

Reactivity: Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Sheep,GuineaPig)

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Sheep,GuineaPig)

1. Sample:
Hcclm3 (Human) Cell Lysate at 40 μg
Lung (Mouse) Lysate at 40 μg
Primary: Anti-NFKB p65 (acetyl-K310) (TMAB-01232) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
2. Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-NFKB p65 (acetyl K310) antibody (TMAB-01232)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated Synthesised acetylpeptide: human NFKBp65 around the acetylation site of Lys310

Human

5970

RelA (p65),NFkB pathway,Inflammatory mediators,RelA (p65),NFkB pathway,Obesity,Host Immune Response,Host Virus Interaction,Alzheimer's disease,NFkB Pathway