Antibody Type: Rabbit Polyclonal

Application: IHC-P,IHC-Fr,ICC/IF,IF,FCM

Reactivity: Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit,Sheep)

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit,Sheep)

1. Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-NF-H antibody (TMAB-01212)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Tissue/cell: SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (NF-H) polyclonal Antibody, Unconjugated (TMAB-01212) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
3. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NF-H Polyclonal Antibody, Unconjugated (TMAB-01212) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

IHC-P,IHC-Fr,ICC/IF,IF,FCM

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1:ug/Test

Polyclonal

KLH conjugated synthetic peptide: human NF-H

Human

4744

Intermediate Filaments,Axon marker,Alzheimer's disease,Neurodegenerative disease,Neurofilaments