Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse,Rat

IgG

Human,Mouse,Rat

1. Sample:
A431 (Human) Cell Lysate at 30 μg
Primary: Anti-Nanog (TMAB-01202) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
2. Sample:
NIH/3T3 (Mouse) Cell Lysate at 30 μg
Primary: Anti-Nanog (TMAB-01202) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Nanog) Polyclonal Antibody, Unconjugated (TMAB-01202) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining.
4. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated (TMAB-01202) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Paraformaldehyde-fixed, paraffin embedded (Mouse ovarian); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Nanog) Polyclonal Antibody, Unconjugated (TMAB-01202) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated (TMAB-01202) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
7. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated (TMAB-01202) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse Nanog

Mouse

71950

Cell differentiation,Surface molecules,SMADs,Transcription Factors,Intracellular