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Anti-Mucin-4/MUC4 Polyclonal Antibody
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Catalog No. TMAB-01187
Anti-Mucin-4/MUC4 Polyclonal Antibody is a Rabbit antibody targeting Mucin-4/MUC4. Anti-Mucin-4/MUC4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Mucin-4/MUC4 Polyclonal Antibody
Anti-Mucin-4/MUC4 Polyclonal Antibody
😃Good
Catalog No. TMAB-01187
Anti-Mucin-4/MUC4 Polyclonal Antibody is a Rabbit antibody targeting Mucin-4/MUC4. Anti-Mucin-4/MUC4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse (predicted:Rat,Pig,Cow,Horse,GuineaPig) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Pig,Cow,Horse,GuineaPig) |
| Verified Activity | 1. Sample: Panc-1 (Human) Cell Lysate at 30 μg LOVO (Human) Cell Lysate at 30 μg Thymus (Mouse) Lysate at 40 μg MKN45 (Human) Cell Lysate at 30 μg Primary: Anti-MUC4 (TMAB-01187) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 120 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Human lung carcinom); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Muc4) Polyclonal Antibody, Unconjugated (TMAB-01187) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human oral squamous cell carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Muc4) Polyclonal Antibody, Unconjugated (TMAB-01187) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass membrane protein (Potential). Secreted. Note=Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing. Mucin-4 beta chain: Cell membrane; Single-pass membrane protein.Mucin-4 alpha chain: Secreted.Isoform 3, 17: Cell membrane; Single-pass membrane protein. Isoform 11, 15: Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human Mucin-4 beta chain |
| Antigen Species | Human |
| Gene ID | 4585 |
| Uniprot ID | |
| Biology Area | MUC,Tumor antigens,Tumor-associated antigens,Defensin,MUC |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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