Anti-Mucin-4/MUC4 Polyclonal Antibody is a Rabbit antibody targeting Mucin-4/MUC4. Anti-Mucin-4/MUC4 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse (predicted:Rat,Pig,Cow,Horse,GuineaPig)

1. Sample:
Panc-1 (Human) Cell Lysate at 30 μg
LOVO (Human) Cell Lysate at 30 μg
Thymus (Mouse) Lysate at 40 μg
MKN45 (Human) Cell Lysate at 30 μg
Primary: Anti-MUC4 (TMAB-01187) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 120 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Human lung carcinom); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Muc4) Polyclonal Antibody, Unconjugated (TMAB-01187) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human oral squamous cell carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Muc4) Polyclonal Antibody, Unconjugated (TMAB-01187) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Mucin-4 beta chain

Human

MUC4

Mucin-4

MUC,Tumor antigens,Tumor-associated antigens,Defensin,MUC

May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation.