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Anti-ME2 Antibody (3E42) is a Mouse antibody targeting ME2. Anti-ME2 Antibody (3E42) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days |
| Description | Anti-ME2 Antibody (3E42) is a Mouse antibody targeting ME2. Anti-ME2 Antibody (3E42) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG1, k |
| Clone | 3E42 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human kidney tissue sections); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ME2) Monoclonal Antibody, Unconjugated (TMAB-08698) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Sample: Lane 1: HL-60 cell lysates Lane 2: NIH/3T3 cell lysates Lane 3: Jurkat cell lysates Primary: Anti-ME2 (TMAB-08698) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Mitochondrion matrix. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] |
| Immunogen | KLH conjugated synthetic peptide: human ME2 |
| Antigen Species | Human |
| Gene Name | ME2 |
| Gene ID | |
| Protein Name | NAD-dependent malic enzyme, mitochondrial |
| Uniprot ID | |
| Function | Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. |
| Molecular Weight | Theoretical: 63 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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