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Anti-ME2 Antibody (3E42)

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Catalog No. TMAB-08698

Anti-ME2 Antibody (3E42) is a Mouse antibody targeting ME2. Anti-ME2 Antibody (3E42) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ME2 Antibody (3E42)

Anti-ME2 Antibody (3E42)

Copy Product Info
😃Good
Catalog No. TMAB-08698
Anti-ME2 Antibody (3E42) is a Mouse antibody targeting ME2. Anti-ME2 Antibody (3E42) can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3747-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-ME2 Antibody (3E42) is a Mouse antibody targeting ME2. Anti-ME2 Antibody (3E42) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG1, k
Clone
3E42
Reactivity
Human,Mouse
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human kidney tissue sections); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ME2) Monoclonal Antibody, Unconjugated (TMAB-08698) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Sample:
Lane 1: HL-60 cell lysates
Lane 2: NIH/3T3 cell lysates
Lane 3: Jurkat cell lysates
Primary: Anti-ME2 (TMAB-08698) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationMitochondrion matrix.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ME2
Antigen Species
Human
Gene Name
ME2
Gene ID
Protein Name
NAD-dependent malic enzyme, mitochondrial
Uniprot ID
Function
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
Chemical Properties
Molecular WeightTheoretical: 63 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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