Anti-MAPT/Tau Antibody (9O262) is a Mouse antibody targeting MAPT/Tau. Anti-MAPT/Tau Antibody (9O262) can be used in IHC-P,IHC-Fr,IF.

IgG2a

9O262

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
8. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4Monoclonal Antibody, Unconjugated (TMAB-08599) at 1:1000 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1: 600 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
11. (Negative control) paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1: 600 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1: 600 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
15. (Negative control) paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08599) at 1: 600 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000

Monoclonal

Recombinant Protein: human Tau-F protein

Human

MAPT

Microtubule-associated protein tau

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.