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Anti-LOX Antibody (2A200)
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Catalog No. TMAC-02414
Alias Protein-lysine 6-oxidase, Lysyl oxidase, LOX
Anti-LOX Antibody (2A200) is a Rabbit antibody targeting LOX. Anti-LOX Antibody (2A200) can be used in WB,ICC,IHC,FCM.
Anti-LOX Antibody (2A200)
Anti-LOX Antibody (2A200)
😃Good
Catalog No. TMAC-02414Alias Protein-lysine 6-oxidase, Lysyl oxidase, LOX
Anti-LOX Antibody (2A200) is a Rabbit antibody targeting LOX. Anti-LOX Antibody (2A200) can be used in WB,ICC,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-LOX Antibody (2A200) is a Rabbit antibody targeting LOX. Anti-LOX Antibody (2A200) can be used in WB,ICC,IHC,FCM. |
| Synonyms | Protein-lysine 6-oxidase, Lysyl oxidase, LOX |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat,zebrafish |
| Verified Activity | 1. Western blot analysis of LOX on different lysates using anti-LOX antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: Mouse brain, Lane 3: Human fetal brain. 2. Western blot analysis of LOX on Zebrafish tissue lysates using anti-LOX antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-LOX antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LOX antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOX antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-LOX antibody. Counter stained with hematoxylin. 7. ICC staining LOX in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining LOX in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining LOX in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of Hela cells with LOX antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-100; ICC: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The lysyl oxidase family of extracellular proteins includes LOX and four LOX-like enzymes, which are responsible for the deamination of peptidyl lysine residues of collagens and elastin. They also catalyze inter- and intra-crosslinking reactions. Overexpression of LOX may cause severe fibrotic degeneration due to its high resistance to degradative enzymes. Procollagen C-proteinase activity processes LOX from a precursor protein to a mature form. Activation of LOX occurs in normal developing and adult skin, and alterations in LOX expression and activity are associated with skin aging and senescence. LOX is crucial for development of the cardiovascular and respiratory systems. In addition, LOX plays a role in cancer, wound healing, as well as cell motility, chemotaxis and differentiation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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