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Anti-LMNB1 Polyclonal Antibody 2

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Catalog No. TMAB-01078

Anti-LMNB1 Polyclonal Antibody 2 is a Rabbit antibody targeting LMNB1. Anti-LMNB1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-LMNB1 Polyclonal Antibody 2

Anti-LMNB1 Polyclonal Antibody 2

😃Good
Catalog No. TMAB-01078
Anti-LMNB1 Polyclonal Antibody 2 is a Rabbit antibody targeting LMNB1. Anti-LMNB1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$373 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,ICC/IF,IF

Reactivity: Rat,Mouse,Human
Ig Type
IgG
Reactivity
Rat,Mouse,Human
Verified Activity
1. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Pancreas (Mouse) Lysate at 40 μg
Lane 3: Testis (Mouse) Lysate at 40 μg
Lane 4: Stomach (Mouse) Lysate at 40 μg
Lane 5: Cerebrum (Rat) Lysate at 40 μg
Lane 7: Testis (Rat) Lysate at 40 μg
Lane 8: Stomach (Rat) Lysate at 40 μg
Lane 9: U937 (Human) Cell Lysate at 30 μg
Lane 10: Jurkat (Human) Cell Lysate at 30 μg
Lane 11: Molt-4 (Human) Cell Lysate at 30 μg
Lane 12: THP-1 (Human) Cell Lysate at 30 μg
Lane 13: Hela (Human) Cell Lysate at 30 μg
Primary:
Anti-Lamin B1 (TMAB-01078) at 1/1000 dilution
Anti-beta-Actin at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67-70 kDa
Observed band size: 65 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Lamin B1) Polyclonal Antibody, Unconjugated (TMAB-01078) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Lamin B1) Polyclonal Antibody, Unconjugated (TMAB-01078) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Lamin B1) Polyclonal Antibody, Unconjugated (TMAB-01078) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus inner membrane; Lipid-anchor; Nucleoplasmic side.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010].
Antigen Details
Immunogen
Recombinant Protein: human Lamin B1
Antigen Species
Human
Gene ID
4001
Uniprot ID
Biology Area
Lamins,Lamins,Lamin B1 - Nuclear,Lamins,Nuclear Envelope
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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