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Anti-LMNB1 Polyclonal Antibody 2 is a Rabbit antibody targeting LMNB1. Anti-LMNB1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $373 | 7-10 days |
Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,ICC/IF,IF Reactivity: Rat,Mouse,Human |
Ig Type | IgG |
Reactivity | Rat,Mouse,Human |
Verified Activity | 1. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Pancreas (Mouse) Lysate at 40 μg Lane 3: Testis (Mouse) Lysate at 40 μg Lane 4: Stomach (Mouse) Lysate at 40 μg Lane 5: Cerebrum (Rat) Lysate at 40 μg Lane 7: Testis (Rat) Lysate at 40 μg Lane 8: Stomach (Rat) Lysate at 40 μg Lane 9: U937 (Human) Cell Lysate at 30 μg Lane 10: Jurkat (Human) Cell Lysate at 30 μg Lane 11: Molt-4 (Human) Cell Lysate at 30 μg Lane 12: THP-1 (Human) Cell Lysate at 30 μg Lane 13: Hela (Human) Cell Lysate at 30 μg Primary: Anti-Lamin B1 (TMAB-01078) at 1/1000 dilution Anti-beta-Actin at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67-70 kDa Observed band size: 65 kDa 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Lamin B1) Polyclonal Antibody, Unconjugated (TMAB-01078) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Lamin B1) Polyclonal Antibody, Unconjugated (TMAB-01078) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Lamin B1) Polyclonal Antibody, Unconjugated (TMAB-01078) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]. |
Immunogen | Recombinant Protein: human Lamin B1 |
Antigen Species | Human |
Gene ID | 4001 |
Uniprot ID | |
Biology Area | Lamins,Lamins,Lamin B1 - Nuclear,Lamins,Nuclear Envelope |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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