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Anti-INTS3 Polyclonal Antibody

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Catalog No. TMAB-07763

Anti-INTS3 Polyclonal Antibody is a Rabbit antibody targeting INTS3. Anti-INTS3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-INTS3 Polyclonal Antibody

Anti-INTS3 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-07763
Anti-INTS3 Polyclonal Antibody is a Rabbit antibody targeting INTS3. Anti-INTS3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-INTS3 Polyclonal Antibody is a Rabbit antibody targeting INTS3. Anti-INTS3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Sample:
Hela (Human) Cell Lysate at 30 μg
A431 (Human) Cell Lysate at 30 μg
Primary: Anti-INTS3 (TMAB-07763) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kD
Observed band size: 118 kD
2. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (INTS3) Polyclonal Antibody, Unconjugated (TMAB-07763) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (INTS3) Polyclonal Antibody, Unconjugated (TMAB-07763) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Note=Localizes to nuclear foci following DNA damage.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundRNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The integrator complex is a multi-protein complex that associates with the C-terminal domain of Pol II and is involved in small nuclear RNA (snRNA) transcription and 3’-end processing. INTS3 (integrator complex subunit 3), also known as INT3 or SOSS-A, is a 1,043 amino acid nuclear protein and is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit and mediates 3-prime end processing of small nuclear RNAs U1 and U2. Expressed in the nucleus, INTS3 exists as four alternatively spliced isoforms and is encoded by a gene located on human chromosome 1q21.3.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human INTS3
Antigen Species
Human
Gene Name
INTS3
Gene ID
Protein Name
Integrator complex subunit 3
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 118 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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