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Anti-INTS3 Polyclonal Antibody
Catalog No. TMAB-07763 Copy Product Info
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Anti-INTS3 Polyclonal Antibody is a Rabbit antibody targeting INTS3. Anti-INTS3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-INTS3 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-07763
Anti-INTS3 Polyclonal Antibody is a Rabbit antibody targeting INTS3. Anti-INTS3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-INTS3 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Bioactivity
Antigen Details
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| Description | Anti-INTS3 Polyclonal Antibody is a Rabbit antibody targeting INTS3. Anti-INTS3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Sample: Hela (Human) Cell Lysate at 30 μg A431 (Human) Cell Lysate at 30 μg Primary: Anti-INTS3 (TMAB-07763) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed band size: 118 kD 2. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (INTS3) Polyclonal Antibody, Unconjugated (TMAB-07763) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (INTS3) Polyclonal Antibody, Unconjugated (TMAB-07763) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Note=Localizes to nuclear foci following DNA damage. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The integrator complex is a multi-protein complex that associates with the C-terminal domain of Pol II and is involved in small nuclear RNA (snRNA) transcription and 3’-end processing. INTS3 (integrator complex subunit 3), also known as INT3 or SOSS-A, is a 1,043 amino acid nuclear protein and is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit and mediates 3-prime end processing of small nuclear RNAs U1 and U2. Expressed in the nucleus, INTS3 exists as four alternatively spliced isoforms and is encoded by a gene located on human chromosome 1q21.3. |
| Immunogen | KLH conjugated synthetic peptide: human INTS3 |
| Antigen Species | Human |
| Gene Name | INTS3 |
| Gene ID | |
| Protein Name | Integrator complex subunit 3 |
| Uniprot ID |
| Molecular Weight | Theoretical: 118 kDa. Actual: 118 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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