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Anti-Insulin Receptor Polyclonal Antibody is a Rabbit antibody targeting Insulin Receptor. Anti-Insulin Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-Insulin Receptor Polyclonal Antibody is a Rabbit antibody targeting Insulin Receptor. Anti-Insulin Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Alias | Insulin Receptor, HHF5, CD220 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Horse,Rabbit,Sheep) |
Verified Activity | 1. Tissue/cell: human liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Insulin Receptor/CD220 Polyclonal Antibody, Unconjugated (TMAB-00974) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Insulin Receptor/CD220 Polyclonal Antibody, Unconjugated (TMAB-00974) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Blank control: Raji (blue). Primary Antibody: Rabbit Anti-Insulin Receptor alpha antibody (TMAB-00974), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (TMAB-00974, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. 4. Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 μg Lane 2: 293T (Human) Cell Lysate at 30 μg Lane 3: LOVO (Human) Cell Lysate at 30 μg Lane 4: Molt-4 (Human) Cell Lysate at 30 μg Lane 5: SW480 (Human) Cell Lysate at 30 μg Lane 6: K562 (Human) Cell Lysate at 30 μg Primary: Anti-Insulin Receptor (TMAB-00974) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kDa Observed band size: 120 kDa 5. Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 μg Lane 2: A549 (Human) Cell Lysate at 30 μg Lane 3: 293T (Human) Cell Lysate at 30 μg Lane 4: Hela (Human) Cell Lysate at 30 μg Lane 5: Liver (Rat) Lysate at 40 μg Lane 6: LympHnode (Mouse) Lysate at 40 μg Lane 7: MOLT-4 (Human) Cell Lysate at 30 μg Lane 8: NIH/3T3 (Mouse) Cell Lysate at 30 μg Primary: Anti-Insulin Receptor (TMAB-00974) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kDa Observed band size: 120 kDa |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Membrane; Single-pass type I membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular. The insulin receptor exhibits receptor tyrosine kinase (RTK) activity. RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates. RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism. Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain containing signaling proteins. |
Immunogen | KLH conjugated synthetic peptide: human Insulin Receptor |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Insulin and insulin-like,Diabetes associated,Diabetes,Heart disease,Metabolism,Insulin / Insulin-like,Receptor Tyrosine Kinases |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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