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Anti-IGFBP-5 Polyclonal Antibody
😃Good
Catalog No. TMAB-00924
Anti-IGFBP-5 Polyclonal Antibody is a Rabbit antibody targeting IGFBP-5. Anti-IGFBP-5 Polyclonal Antibody can be used in WB.
Anti-IGFBP-5 Polyclonal Antibody
Anti-IGFBP-5 Polyclonal Antibody
😃Good
Catalog No. TMAB-00924
Anti-IGFBP-5 Polyclonal Antibody is a Rabbit antibody targeting IGFBP-5. Anti-IGFBP-5 Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB Reactivity: Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Sheep) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. Sample: Kidney (Mouse) Lysate at 40 μg Primary: Anti-IGFBP5 (TMAB-00924) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa 2. Tissue/cell: human nasopharyngeal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-IGFBP5/IBP5 Polyclonal Antibody, Unconjugated (TMAB-00924) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| Application | WB |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development. Function : IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. |
| Immunogen | KLH conjugated synthetic peptide: human IGFBP5 |
| Antigen Species | Human |
| Gene ID | 3488 |
| Uniprot ID | |
| Biology Area | Insulin / Insulin-like,Lipid Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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