Anti-ICAM5 Polyclonal Antibody is a Rabbit antibody targeting ICAM5. Anti-ICAM5 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Pig,Cow)

1. Sample: Hippocampus (Mouse) Lysate at 40 μg
Primary: Anti-ICAM5 (TMAB-07478) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 97 kD
Observed band size: 167 kD
2. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-ICAM5 antibody (TMAB-07478)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 0.2 μg /test.
Protocol
The cells were incubated in 5 %BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 0.2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human ICAM5

Human

ICAM5

Intercellular adhesion molecule 5

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2).