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Anti-Iba1 Antibody (1X229)

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Catalog No. TMAC-02080
Alias IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1

Anti-Iba1 Antibody (1X229) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (1X229) can be used in WB,ICC,IF,IHC,FCM,IP.

Anti-Iba1 Antibody (1X229)

Anti-Iba1 Antibody (1X229)

😃Good
Catalog No. TMAC-02080Alias IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1
Anti-Iba1 Antibody (1X229) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (1X229) can be used in WB,ICC,IF,IHC,FCM,IP.
Pack SizePriceAvailabilityQuantity
50 μL$2967-10 days
100 μL$4987-10 days
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Product Introduction

Bioactivity
Description
Anti-Iba1 Antibody (1X229) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (1X229) can be used in WB,ICC,IF,IHC,FCM,IP.
SynonymsIRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Iba1 antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Iba1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Iba1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Iba1 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Iba1 antibody. Counter stained with hematoxylin.
6. ICC staining Iba1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of THP-1 cells with Iba1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
8. All lanes : Iba1 Antibody at 1/2k dilution, Lane 1: Mouse liver lysates, Lane 2: Mouse spleen lysates, Lane 3: Mouse lung lysates, Lane 4: Rat liver lysates, Lane 5: Rat spleen lysates, Lane 6: Rat lung lysates Lysates/proteins at 20 μg per lane. Secondary All lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution, Predicted band size: 17 kDa, Observed band size: 17 kDa, Exposure time: 120 seconds.
Application
Recommended Dose
WB: 1:500-1000; IHC: 1:100-500; ICC:IF: 1:50-100; IP: 1:10-50; FCM: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundIonized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 17 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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