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Anti-GRP78/Bip Antibody (4S162)
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Catalog No. TMAB-06799
Anti-GRP78/Bip Antibody (4S162) is a Mouse antibody targeting GRP78/Bip. Anti-GRP78/Bip Antibody (4S162) can be used in WB,ICC/IF.
Anti-GRP78/Bip Antibody (4S162)
Anti-GRP78/Bip Antibody (4S162)
Copy Product Info😃Good
Catalog No. TMAB-06799
Anti-GRP78/Bip Antibody (4S162) is a Mouse antibody targeting GRP78/Bip. Anti-GRP78/Bip Antibody (4S162) can be used in WB,ICC/IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 200 μg | $1,060 | 7-10 days | 7-10 days | |
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-GRP78/Bip Antibody (4S162) is a Mouse antibody targeting GRP78/Bip. Anti-GRP78/Bip Antibody (4S162) can be used in WB,ICC/IF. |
| Ig Type | IgG |
| Clone | 4S162 |
| Reactivity | Human (predicted:Rat) |
| Verified Activity | 1. Sample: U87Mg (Human) Cell Lysate at 30 μg Hela (Human) Cell Lysate at 30 μg HepG2 (Human) Cell Lysate at 30 μg MDA-MB-231 (Human) Cell Lysate at 30 μg U251 (Human) Cell Lysate at 30 μg U2os (Human) Cell Lysate at 30 μg Primary: Anti-GRP78/Bip (TMAB-06799) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 78 kD 2. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (GRP78/Bip) monoclonol Antibody, Unconjugated (TMAB-06799) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
| Application | |
| Recommended Dose | WB: 1:500-1000; ICC/IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. |
| Immunogen | KLH conjugated synthetic peptide: human GRP78/Bip |
| Antigen Species | Human |
| Gene Name | HSPA5 |
| Gene ID | |
| Protein Name | Endoplasmic reticulum chaperone BiP |
| Uniprot ID | |
| Function | Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. |
| Molecular Weight | Theoretical: 78 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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