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Anti-GPI Antibody (7B780)

Catalog No. TMAB-06669 Copy Product Info
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Anti-GPI Antibody (7B780) is a Mouse antibody targeting GPI. Anti-GPI Antibody (7B780) can be used in WB,ICC/IF.

Anti-GPI Antibody (7B780)

Copy Product Info
🥰Excellent
Catalog No. TMAB-06669

Anti-GPI Antibody (7B780) is a Mouse antibody targeting GPI. Anti-GPI Antibody (7B780) can be used in WB,ICC/IF.

Anti-GPI Antibody
(7B780)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1517-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4707-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GPI Antibody (7B780) is a Mouse antibody targeting GPI. Anti-GPI Antibody (7B780) can be used in WB,ICC/IF.
Ig Type
IgG1, Kappa
Clone
7B780
Reactivity
Human (predicted:Rat)
Verified Activity
1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:10000
Primary ab incubation condition: room temperature 2 h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, HepG2, Rat kidney
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 63 kDa
Observed MW: 63 kDa
2. Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-06669
Application
Recommended Dose
WB: 1:2000-10000; ICC/IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasm. Secreted.
ConstructionRecombinant Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq, Jul 2008].
Antigen Details
Gene Name
GPI
Gene ID
Protein Name
Glucose-6-phosphate isomerase
Function
Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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