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Anti-GJA1 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00773
Anti-GJA1 Polyclonal Antibody 2 is a Rabbit antibody targeting GJA1. Anti-GJA1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-GJA1 Polyclonal Antibody 2
Anti-GJA1 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00773
Anti-GJA1 Polyclonal Antibody 2 is a Rabbit antibody targeting GJA1. Anti-GJA1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,FCM Reactivity: Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit) |
| Verified Activity | 1. Tissue/cell: human cervix carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Connexin 43 Polyclonal Antibody, Unconjugated (TMAB-00773) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Connexin 43 Polyclonal Antibody, Unconjugated (TMAB-00773) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Blank control (blue): HUVEC cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Connexin 43 antibody (TMAB-00773), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 4. Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Rat Heart tissue lysates Lane 3: Mouse Cerebrum tissue lysates Lane 4: Rat Cerebrum tissue lysates Lane 5: Mouse Stomach tissue lysates Primary: Anti-Connexin 43 (TMAB-00773) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 48 kDa |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human Connexin 43 |
| Antigen Species | Human |
| Gene ID | 2697 |
| Uniprot ID | |
| Biology Area | Cell junction molecules,Cardiac arrhythmias,Gap Junctions |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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