Antibody Type: Rabbit Polyclonal

Application: IHC-P,IHC-Fr,IF,FCM

Reactivity: Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,GuineaPig)

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,GuineaPig)

1. Tissue/cell: human meningioma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GATA3 Polyclonal Antibody, Unconjugated (TMAB-00748) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-GATA3 antibody (TMAB-00748)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

IHC-P,IHC-Fr,IF,FCM

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/test

Polyclonal

KLH conjugated synthetic peptide: human GATA3

Human

2625

Ectoderm,ChIP antibodies,Zinc Finger,Non-CD,Ectoderm