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Anti-Fbx32 Antibody (4A434)
😃Good
Catalog No. TMAB-00661
Anti-Fbx32 Antibody (4A434) is a Rabbit antibody targeting Fbx32. Anti-Fbx32 Antibody (4A434) can be used in WB,ICC/IF.
Anti-Fbx32 Antibody (4A434)
Anti-Fbx32 Antibody (4A434)
😃Good
Catalog No. TMAB-00661
Anti-Fbx32 Antibody (4A434) is a Rabbit antibody targeting Fbx32. Anti-Fbx32 Antibody (4A434) can be used in WB,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $153 | 7-10 days | |
| 50 μL | $273 | 7-10 days | |
| 100 μL | $489 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,ICC/IF Reactivity: Mouse,Rat (predicted:Human) |
| Ig Type | IgG |
| Clone | 4A434 |
| Reactivity | Mouse,Rat (predicted:Human) |
| Verified Activity | 25 μg total protein per Lane of various lysates probed with Fbx32 monoclonal antibody, unconjugated (TMAB-00661) at 1:10000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| Application | WB,ICC/IF |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Note=Shuttles between Cytoplasm and the nucleus. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Fbx32 is an E3 ubiquitin ligase that initiates ATP dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. It is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. It is also thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Fbx32 interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. |
| Immunogen | A synthesized peptide: human FBXO32 |
| Antigen Species | Human |
| Gene ID | 114907 |
| Uniprot ID | |
| Biology Area | Hypertrophy,F Box E3 Ligase,SCF Complex E3 Ligase,Myogenesis |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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