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Anti-FAF1 Polyclonal Antibody

Catalog No. TMAB-05804 Copy Product Info
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Anti-FAF1 Polyclonal Antibody is a Rabbit antibody targeting FAF1. Anti-FAF1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-FAF1 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-05804

Anti-FAF1 Polyclonal Antibody is a Rabbit antibody targeting FAF1. Anti-FAF1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-FAF1 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2237-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-FAF1 Polyclonal Antibody is a Rabbit antibody targeting FAF1. Anti-FAF1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Rat (predicted:Human,Mouse,Chicken,Dog,Cow,Rabbit)
Verified Activity
1. Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-FAF1 Polyclonal Antibody, Unconjugated (TMAB-05804) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAF1) Polyclonal Antibody, Unconjugated (TMAB-05804) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
Tissue SpecificityMost abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon. Not detected in the peripheral blood leukocytes.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundFAF1 (Fas-associated protein factor 1) specifically interacts with the cytoplasmic domain of wild type, but not mutated, Fas in both yeast and mammalian cells. It is transiently expressed in T cells and potentiates Fas-induced apoptosis. FAF1 lacks a DDH (Death domain homologous region), in contrast to TRADD, FADD, and RIP, and cannot induce apoptosis independent of Fas activation. Fas associated factor 1; CGI ; FAF1 protein; Fas (TNFRSF6) associated factor 1; FAS associated factor 1; FLJ37524; hFAF1; HFAF1s; Protein FAF1; TNFRSF6 associated factor 1.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human FAF1
Antigen Species
Human
Gene Name
FAF1
Gene ID
Protein Name
FAS-associated factor 1
Uniprot ID
Function
Potentiates but cannot initiate FAS-induced apoptosis.
Chemical Properties
Molecular WeightTheoretical: 74 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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