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Anti-EPOR Polyclonal Antibody
😃Good
Catalog No. TMAB-00628
Anti-EPOR Polyclonal Antibody is a Rabbit antibody targeting EPOR. Anti-EPOR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-EPOR Polyclonal Antibody
Anti-EPOR Polyclonal Antibody
😃Good
Catalog No. TMAB-00628
Anti-EPOR Polyclonal Antibody is a Rabbit antibody targeting EPOR. Anti-EPOR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-EPOR Polyclonal Antibody is a Rabbit antibody targeting EPOR. Anti-EPOR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Cow,Horse) |
| Verified Activity | 1. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-EPOR Polyclonal Antibody, Unconjugated (TMAB-00628) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-EPOR Polyclonal Antibody, Unconjugated (TMAB-00628) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: K562 (Human) Cell Lysate at 30 μg Primary: Anti-EPOR (TMAB-00628) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 64 kDa 4. Sample: K562 (Human) Cell Lysate at 30 μg Primary: Anti-EPOR (TMAB-00628) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 30/52 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Isoform EPOR-S: Secreted. Note=Secreted and located to the cell surface. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The erythropoietin receptor (EPOR) is a member of the cytokine receptor family. There are several isoforms including: EPOR-F (full length), EPOR-S (soluble form), and EPOR-T (truncated form). Upon erythropoietin (EPO) binding, the EPOR activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated EPOR appears to have a role in erythroid cell survival. Defects in the EPOR may produce erythroleukemia and familial erythrocytosis. A functional EPOR is found in the cardiovascular system, including endothelial cells and cardiomyocytes, and data suggest that the EPO/EPO receptor system plays an important role in cardiac function. In animal studies, treatment with EPO during ischemia/reperfusion in the heart has been shown to limit the infarct size and the extent of apoptosis. |
| Immunogen | KLH conjugated synthetic peptide: human EPOR |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Angiogenic Factors,Endothelial Cell Markers,Ischemia / Reperfusion,Apoptosis,Hypertrophy,Receptors,Small G Proteins,Surface Molecules |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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