Anti-EGFR Antibody (2W575) is a Rabbit antibody targeting EGFR. Anti-EGFR Antibody (2W575) can be used in WB,ICC/IF,IHC,IP,FCM.

IgG

Human,Mouse,Rat

1. Western blot analysis of EGFR on different lysates using anti-EGFR antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293T, Lane 3: Human skin.
2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-EGFR antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EGFR antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-EGFR antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EGFR antibody. Counter stained with hematoxylin.
6. ICC staining EGFR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining EGFR in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining EGFR in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of A431 cells with EGFR antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100

Monoclonal

Unconjugated

Recombinant Protein