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Anti-EGF Polyclonal Antibody 2
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Catalog No. TMAB-00592
Anti-EGF Polyclonal Antibody 2 is a Rabbit antibody targeting EGF. Anti-EGF Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.
Anti-EGF Polyclonal Antibody 2
Anti-EGF Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00592
Anti-EGF Polyclonal Antibody 2 is a Rabbit antibody targeting EGF. Anti-EGF Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: IHC-P,IHC-Fr,IF Reactivity: Rat |
| Ig Type | IgG |
| Reactivity | Rat |
| Verified Activity | 1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-EGf (rat) Polyclonal Antibody, Unconjugated (TMAB-00592) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EGF) Polyclonal Antibody, Unconjugated (TMAB-00592) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| Application | IHC-P,IHC-Fr,IF |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid protein originally found in rodents and humans. It has been shown to be a potent mitogen for a variety of cell types both in vivo and in vitro. EGF binds to the EGF receptor on the surface of cells and mediates intrinsic phosphorylation of the receptor on tyrosine residues. It has been detected in nearly all body fluids, such as urine (urogastrone), saliva, milk and platelet-rich plasma. EGF, TGF?and vaccinia virus growth factor exhibit 30-40% amino acid homology. Several additional members of the EGF/TGF family have been described; these include Cripto, Amphiregulin and the heparin-binding EGF-like growth factor. Amphiregulin and the heparin-binding EGF-like growth factor both bind to the EGF receptor. |
| Immunogen | KLH conjugated synthetic peptide: rat EGF |
| Antigen Species | Rat |
| Biology Area | EGF,Cell differentiation,Growth factors and hormones ELISA kits,EGF |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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