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Anti-Drosha Antibody (5J107)
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Catalog No. TMAC-01185
Alias RNC_HUMAN, RNASEN, RNaseIII, RNASE3L, RNase3, RNase III, RNase 3, RN3, RN 3, RibonucleaseIII, Ribonuclease type III nuclear, Ribonuclease III nuclear, Ribonuclease III, Ribonuclease 3, RANSE3L, Putative ribonuclease III, Putative protein p241 which interacts with transcription factor Sp1, Protein Drosha, p241, Nuclear RNase III Drosha, HSA242976, Etohi2, Drosha ribonuclease type III, Drosha double stranded RNA specific endoribonuclease, DROSHA
Anti-Drosha Antibody (5J107) is a Rabbit antibody targeting Drosha. Anti-Drosha Antibody (5J107) can be used in WB,ICC,IF,IHC,FCM.
Anti-Drosha Antibody (5J107)
Anti-Drosha Antibody (5J107)
😃Good
Catalog No. TMAC-01185Alias RNC_HUMAN, RNASEN, RNaseIII, RNASE3L, RNase3, RNase III, RNase 3, RN3, RN 3, RibonucleaseIII, Ribonuclease type III nuclear, Ribonuclease III nuclear, Ribonuclease III, Ribonuclease 3, RANSE3L, Putative ribonuclease III, Putative protein p241 which interacts with transcription factor Sp1, Protein Drosha, p241, Nuclear RNase III Drosha, HSA242976, Etohi2, Drosha ribonuclease type III, Drosha double stranded RNA specific endoribonuclease, DROSHA
Anti-Drosha Antibody (5J107) is a Rabbit antibody targeting Drosha. Anti-Drosha Antibody (5J107) can be used in WB,ICC,IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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| Description | Anti-Drosha Antibody (5J107) is a Rabbit antibody targeting Drosha. Anti-Drosha Antibody (5J107) can be used in WB,ICC,IF,IHC,FCM. |
| Synonyms | RNC_HUMAN, RNASEN, RNaseIII, RNASE3L, RNase3, RNase III, RNase 3, RN3, RN 3, RibonucleaseIII, Ribonuclease type III nuclear, Ribonuclease III nuclear, Ribonuclease III, Ribonuclease 3, RANSE3L, Putative ribonuclease III, Putative protein p241 which interacts with transcription factor Sp1, Protein Drosha, p241, Nuclear RNase III Drosha, HSA242976, Etohi2, Drosha ribonuclease type III, Drosha double stranded RNA specific endoribonuclease, DROSHA |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Drosha on Hela cell and SiHa cell lysates using anti-Drosha antibody at 1/500 dilution. 2. Western blot analysis of Drosha on K562 cell lysates using anti-Drosha antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Drosha antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Drosha antibody. Counter stained with hematoxylin. 5. ICC staining Drosha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Drosha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Drosha in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Jurkat cells with Drosha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The ribonuclease III superfamily represents a structurally distinct group of double-strand-specific endonucleases with essential roles in RNA maturation, RNA decay, and gene silencing. Initial cleavage of microRNAs is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus. Human Drosha is a component of two multi-protein complexes. The larger complex contains multiple classes of RNA-associated proteins including RNA helicases, proteins that bind double-stranded RNA, novel heterogeneous nuclear ribonucleoproteins and the Ewing's sarcoma family of proteins. The smaller complex is composed of Drosha and the double-stranded-RNA-binding protein, DGCR8. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 159 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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