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Anti-CYP7A1 Polyclonal Antibody 2 is a Rabbit antibody targeting CYP7A1. Anti-CYP7A1 Polyclonal Antibody 2 can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Antibody Type: Rabbit Polyclonal Application: WB Reactivity: Human,Mouse (predicted:Rat) |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat) |
Verified Activity | 1. Sample: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-CYP7A1 (TMAB-00526) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa 2. Sample: Liver (Mouse) Lysate at 40 μg Primary: Anti-CYP7A1 (TMAB-00526) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa |
Application | WB |
Recommended Dose | WB: 1:500-2000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]. |
Immunogen | KLH conjugated synthetic peptide: mouse CYP7A1 |
Antigen Species | Mouse |
Gene ID | 13122 |
Uniprot ID | |
Biology Area | Lipid metabolism,Cancer,Cytochromes,Lipid metabolism,Lipases,Cholesterol Metabolism,Cytochromes,Cholesterol Metabolism,Metabolism of lipids and lipoproteins |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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