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Anti-CSPG4 Polyclonal Antibody

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Catalog No. TMAB-00488
Alias Melanoma-associated chondroitin sulfate proteoglycan, Melanoma chondroitin sulfate proteoglycan, MCSP, CSPG4, Chondroitin sulfate proteoglycan NG2, Chondroitin sulfate proteoglycan 4

Anti-CSPG4 Polyclonal Antibody is a Rabbit antibody targeting CSPG4. Anti-CSPG4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CSPG4 Polyclonal Antibody

Anti-CSPG4 Polyclonal Antibody

😃Good
Catalog No. TMAB-00488Alias Melanoma-associated chondroitin sulfate proteoglycan, Melanoma chondroitin sulfate proteoglycan, MCSP, CSPG4, Chondroitin sulfate proteoglycan NG2, Chondroitin sulfate proteoglycan 4
Anti-CSPG4 Polyclonal Antibody is a Rabbit antibody targeting CSPG4. Anti-CSPG4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3737-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-CSPG4 Polyclonal Antibody is a Rabbit antibody targeting CSPG4. Anti-CSPG4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasMelanoma-associated chondroitin sulfate proteoglycan, Melanoma chondroitin sulfate proteoglycan, MCSP, CSPG4, Chondroitin sulfate proteoglycan NG2, Chondroitin sulfate proteoglycan 4
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Pig,Horse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NG2) Polyclonal Antibody, Unconjugated (TMAB-00488) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Hippocampus (Mouse) Lysate at 40 μg
Primary: Anti-NG2 (TMAB-00488) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 247 kDa
Observed band size: 350 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NG2) Polyclonal Antibody, Unconjugated (TMAB-00488) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationApical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundProteoglycans (PGs) are a family of proteins composed of different polypeptide chains containing glycosaminoglycan (GAG) modifications. They vary in their cellular locations and have diverse structure and functions. NG2 is a proteoglycan found in the nervous system, comprising a large integral membrane PG with a core protein of 300 kDa and at least one covalently attached chondroitin sulfate (CS) GAG chain. In the CNS this protein is expressed mainly on the surfaces of developing and adult oligodendrocyte precursor cells, but is also associated with developing chondrocytes, cardiomyocytes, pericytes and several human tumours. NG2 also stimulates alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NG2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Tumor Associated,Neurogenesis,Oligodendrocyte marker,Integrins
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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