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Anti-CSPG4 Polyclonal Antibody
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Catalog No. TMAB-00488
Alias Melanoma-associated chondroitin sulfate proteoglycan, Melanoma chondroitin sulfate proteoglycan, MCSP, CSPG4, Chondroitin sulfate proteoglycan NG2, Chondroitin sulfate proteoglycan 4
Anti-CSPG4 Polyclonal Antibody is a Rabbit antibody targeting CSPG4. Anti-CSPG4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CSPG4 Polyclonal Antibody
Anti-CSPG4 Polyclonal Antibody
😃Good
Catalog No. TMAB-00488Alias Melanoma-associated chondroitin sulfate proteoglycan, Melanoma chondroitin sulfate proteoglycan, MCSP, CSPG4, Chondroitin sulfate proteoglycan NG2, Chondroitin sulfate proteoglycan 4
Anti-CSPG4 Polyclonal Antibody is a Rabbit antibody targeting CSPG4. Anti-CSPG4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CSPG4 Polyclonal Antibody is a Rabbit antibody targeting CSPG4. Anti-CSPG4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Melanoma-associated chondroitin sulfate proteoglycan, Melanoma chondroitin sulfate proteoglycan, MCSP, CSPG4, Chondroitin sulfate proteoglycan NG2, Chondroitin sulfate proteoglycan 4 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Pig,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NG2) Polyclonal Antibody, Unconjugated (TMAB-00488) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Hippocampus (Mouse) Lysate at 40 μg Primary: Anti-NG2 (TMAB-00488) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 247 kDa Observed band size: 350 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NG2) Polyclonal Antibody, Unconjugated (TMAB-00488) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Proteoglycans (PGs) are a family of proteins composed of different polypeptide chains containing glycosaminoglycan (GAG) modifications. They vary in their cellular locations and have diverse structure and functions. NG2 is a proteoglycan found in the nervous system, comprising a large integral membrane PG with a core protein of 300 kDa and at least one covalently attached chondroitin sulfate (CS) GAG chain. In the CNS this protein is expressed mainly on the surfaces of developing and adult oligodendrocyte precursor cells, but is also associated with developing chondrocytes, cardiomyocytes, pericytes and several human tumours. NG2 also stimulates alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. |
| Immunogen | KLH conjugated synthetic peptide: human NG2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Tumor Associated,Neurogenesis,Oligodendrocyte marker,Integrins |
| Molecular Weight | Theoretical: 247 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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