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Anti-CRTAM Antibody-APC (2G306)
🥰Excellent
Catalog No. TMAY-02175A
Anti-CRTAM Antibody-APC (2G306) is a APC-conjugated Mouse antibody targeting CRTAM. Anti-CRTAM Antibody-APC (2G306) can be used in FCM.
Anti-CRTAM Antibody-APC (2G306)
Anti-CRTAM Antibody-APC (2G306)
🥰Excellent
Catalog No. TMAY-02175A
Anti-CRTAM Antibody-APC (2G306) is a APC-conjugated Mouse antibody targeting CRTAM. Anti-CRTAM Antibody-APC (2G306) can be used in FCM.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CRTAM Antibody-APC (2G306) is a APC-conjugated Mouse antibody targeting CRTAM. Anti-CRTAM Antibody-APC (2G306) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 2G306 |
| Reactivity | Human |
| Verified Activity | Flow cytometric analysis of Human CRTAM(CD355) expression on human peripheral blood lymphocytes. Human peripheral blood mononulcear cells were stimulated for 24 hours with PMA and Ionomycin, then stained with APC-conjugated anti-Human CRTAM. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. |
| Application | FCM |
| Recommended Dose | 5 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CRTAM (rh CRTAM; TMPY-01674; NP_062550.2; Met1-Ser286) and conjugated with APC under optimum conditions, the unreacted APC was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| Research Background | Cytotoxic and regulatory T-cell molecule, also known as Class-I MHC-restricted T-cell-associated molecule and CRTAM, is a single-pass type I membrane protein which belongs to thenectin family.CRTAM contains oneIg-like C2-type (immunoglobulin-like) domain and oneIg-like V-type (immunoglobulin-like) domain. In the immune system, the expression of CRTAM is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. It is strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in purkinje neurons in cerebellum. It is expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues. CRTAM is a member of the immunoglobulin superfamily that complies with the structural characteristics of the JAM family of proteins and is phylogenetically more closely related to nectin-like proteins. It is a molecule involved in epithelial cell adhesion. CRTAM is sensitive to intermediate filament disruption and treatment of monolayers with soluble CRTAM enhances cell-cell dissociation and lowers transepithelial electrical resistance. CRTAM may also induce retention by binding to CD8+ dendritic cells (DCs) at the late stage of activation before proliferation. |
| Conjucates | APC |
| Others Formats | Unconjugated/PE |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Human CRTAM Protein (TMPY-01674) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| Transport | Shipping with blue ice. |
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