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Anti-CREB1 Antibody (3J147)
😃Good
Catalog No. TMAB-00478
Anti-CREB1 Antibody (3J147) is a Mouse antibody targeting CREB1. Anti-CREB1 Antibody (3J147) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CREB1 Antibody (3J147)
Anti-CREB1 Antibody (3J147)
😃Good
Catalog No. TMAB-00478
Anti-CREB1 Antibody (3J147) is a Mouse antibody targeting CREB1. Anti-CREB1 Antibody (3J147) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse |
| Ig Type | IgG |
| Clone | 3J147 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse embryos tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CREB-1) Monoclonal Antibody, Unconjugated (ascites of TMAB-00478) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: Jurkat (Human) Cell Lysate at 30 μg Huvec (Human) Cell Lysate at 30 μg U2os (Human) Cell Lysate at 30 μg Primary: Anti-CREB-1 (TMAB-00478) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 45 kDa Observed band size: 43 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CREB-1) Monoclonal Antibody, Unconjugated (ascites of TMAB-00478) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CREB-1) Monoclonal Antibody, Unconjugated (ascites of TMAB-00478) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 5. Sample: NIH/3T3 Cell (Mouse) Lysate at 40 μg Primary: Anti-CREB-1 (TMAB-00478) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 45 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Nucleus. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The ATF/CREB family consists of transcription factors that function through binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2, ATF-1,ATF-2,ATF-3and ATF-4. these transcription factors share highly-related COOH terminal leucine zipper demerization and basic DNA bindings but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins bind CREs in their homodimeric form, in cerain instances they also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. It has recentlybeen shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265kDa nuclear protein designated CBP (CREB-binding protein), which may reprecent a CREB co-activator. |
| Immunogen | Recombinant Protein: human CREB-1 |
| Antigen Species | Human |
| Gene ID | 1385 |
| Uniprot ID | |
| Biology Area | HLH / Leucine Zipper,Transcription Factors,TLR Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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