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Anti-Cingulin Polyclonal Antibody
😃Good
Catalog No. TMAB-04357
Anti-Cingulin Polyclonal Antibody is a Rabbit antibody targeting Cingulin. Anti-Cingulin Polyclonal Antibody can be used in WB.
Anti-Cingulin Polyclonal Antibody
Anti-Cingulin Polyclonal Antibody
😃Good
Catalog No. TMAB-04357
Anti-Cingulin Polyclonal Antibody is a Rabbit antibody targeting Cingulin. Anti-Cingulin Polyclonal Antibody can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Cingulin Polyclonal Antibody is a Rabbit antibody targeting Cingulin. Anti-Cingulin Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Cow,Horse,Sheep) |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell junction > tight junction. Localizes to the apical junction complex composed of tight and adherens junctions. |
| Tissue Specificity | Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Diffusion of solutes is prevented across certain barriers by the formation of tight junction seals. Occludin and Cingulin interact with other proteins to direct the formation and regulation of tight junctions. Cingulin, a protein component of the submembrane plaque of tight junctions (TJ), contains globular and coiled-coil domains and interacts in vitro with several TJ and cytoskeletal proteins, including the PDZ protein ZO-1. Cingulin binding has also been shown to inhibit RhoA activation and signaling with increased Cingulin expression in confluent cells, causing downregulation of RhoA by inhibiting GEF-H1/Lfc. |
| Immunogen | KLH conjugated synthetic peptide: human Cingulin |
| Antigen Species | Human |
| Gene Name | CGN |
| Gene ID | |
| Protein Name | Cingulin |
| Uniprot ID | |
| Function | Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. |
| Molecular Weight | Theoretical: 136 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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