Anti-CHRM4 Polyclonal Antibody 3 is a Rabbit antibody targeting CHRM4. Anti-CHRM4 Polyclonal Antibody 3 can be used in FCM.

IgG

Human (predicted:Mouse,Rat)

Black line : Positive blank control A431); Negative blank control (Huvec)
Green line : Primary Antibody (Rabbit Anti-CHRM4 antibody (TMAB-04323))
Orange line：Isotype Control Antibody (Rabbit IgG).
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488)
A431 (Positive) and HUVEC Negative control) Cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CHRM4 antibody (TMAB-04323) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibodY (blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CHRM4/mAChR M4

Human

CHRM4

Muscarinic acetylcholine receptor M4

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and postassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus.