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Anti-CHEK1 Antibody (6N369)
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Catalog No. TMAC-00839
Alias Serine/threonine-protein kinase Chk1, EC 2.7.11.1, CHK1 checkpoint homolog, CHK1, CHEK 1, Checkpoint kinase-1, Cell cycle checkpoint kinase
Anti-CHEK1 Antibody (6N369) is a Rabbit antibody targeting CHEK1. Anti-CHEK1 Antibody (6N369) can be used in WB,ICC/IF,IHC,FCM.
Anti-CHEK1 Antibody (6N369)
Anti-CHEK1 Antibody (6N369)
😃Good
Catalog No. TMAC-00839Alias Serine/threonine-protein kinase Chk1, EC 2.7.11.1, CHK1 checkpoint homolog, CHK1, CHEK 1, Checkpoint kinase-1, Cell cycle checkpoint kinase
Anti-CHEK1 Antibody (6N369) is a Rabbit antibody targeting CHEK1. Anti-CHEK1 Antibody (6N369) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-CHEK1 Antibody (6N369) is a Rabbit antibody targeting CHEK1. Anti-CHEK1 Antibody (6N369) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | Serine/threonine-protein kinase Chk1, EC 2.7.11.1, CHK1 checkpoint homolog, CHK1, CHEK 1, Checkpoint kinase-1, Cell cycle checkpoint kinase |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Chk1 on different lysates using anti-Chk1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: PC-3M. 2. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Chk1 antibody. Counter stained with hematoxylin. 3. ICC staining Chk1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Chk1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Chk1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with Chk1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 54 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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