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Anti-CH25H Polyclonal Antibody 2
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Catalog No. TMAB-00422
Anti-CH25H Polyclonal Antibody 2 is a Rabbit antibody targeting CH25H. Anti-CH25H Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CH25H Polyclonal Antibody 2
Anti-CH25H Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00422
Anti-CH25H Polyclonal Antibody 2 is a Rabbit antibody targeting CH25H. Anti-CH25H Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Rat |
| Ig Type | IgG |
| Reactivity | Human,Rat |
| Verified Activity | Sample: Lane 1: Human U-87 MG cell Lysates Primary: Anti-CH25H (TMAB-00422) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum membrane |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | CH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. |
| Immunogen | KLH conjugated synthetic peptide: human CH25H |
| Antigen Species | Human |
| Gene ID | 9023 |
| Uniprot ID | |
| Biology Area | Metabolism of lipids and lipoproteins,Cholesterol Metabolism,Oxidative Stress,Cholesterol Metabolism,Lipid metabolism,Oxidative stress |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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