Anti-CEP55 Polyclonal Antibody is a Rabbit antibody targeting CEP55. Anti-CEP55 Polyclonal Antibody can be used in WB,FCM.

IgG

Mouse (predicted:Human,Rat,Dog,Pig,Horse,Sheep)

1. Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-CEP55 antibody (TMAB-04249)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Testis (Mouse) Lysate at 40 μg
Primary: Anti-CEP55 (TMAB-04249) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 52 kD

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CEP55

Human

CEP55

Centrosomal protein of 55 kDa

Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.