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Anti-CDK4 Antibody (1F732)
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Catalog No. TMAC-00792
Alias PSK-J3, cyclin-dependent kinase 4, CMM3
Anti-CDK4 Antibody (1F732) is a Rabbit antibody targeting CDK4. Anti-CDK4 Antibody (1F732) can be used in FCM,ICC/IF,IHC,WB.
Anti-CDK4 Antibody (1F732)
Anti-CDK4 Antibody (1F732)
😃Good
Catalog No. TMAC-00792Alias PSK-J3, cyclin-dependent kinase 4, CMM3
Anti-CDK4 Antibody (1F732) is a Rabbit antibody targeting CDK4. Anti-CDK4 Antibody (1F732) can be used in FCM,ICC/IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-CDK4 Antibody (1F732) is a Rabbit antibody targeting CDK4. Anti-CDK4 Antibody (1F732) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | PSK-J3, cyclin-dependent kinase 4, CMM3 |
| Ig Type | IgG |
| Clone | 1F732 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Cdk4 on Hela cells lysates using anti-Cdk4 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk4 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cdk4 antibody. Counter stained with hematoxylin. 4. ICC staining Cdk4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Cdk4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Cdk4 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of MCF-7 cells with Cdk4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression . Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK). |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 34 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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