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Anti-CD300A Antibody-APC (9F620)
🥰Excellent
Catalog No. TMAY-02353A
Anti-CD300A Antibody-APC (9F620) is a APC-conjugated Mouse antibody targeting CD300A. Anti-CD300A Antibody-APC (9F620) can be used in FCM.
Anti-CD300A Antibody-APC (9F620)
Anti-CD300A Antibody-APC (9F620)
🥰Excellent
Catalog No. TMAY-02353A
Anti-CD300A Antibody-APC (9F620) is a APC-conjugated Mouse antibody targeting CD300A. Anti-CD300A Antibody-APC (9F620) can be used in FCM.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CD300A Antibody-APC (9F620) is a APC-conjugated Mouse antibody targeting CD300A. Anti-CD300A Antibody-APC (9F620) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 9F620 |
| Reactivity | Human |
| Verified Activity | Flow cytometric analysis of human CD300A expression on human whole blood monocytes. Human whole blood monocytes were stained with APC-conjugated anti-Human CD300A. The histogram were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. |
| Application | FCM |
| Recommended Dose | 5 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD300A (rh CD300A; TMPY-02013; Q9UGN4-1; Met1-Gln178) and conjugated with APC under optimum conditions, the unreacted APC was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| Research Background | CMRF35-like molecule 8, also known as CD300 antigen-like family member A, CMRF35-H9, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CD300a and CMRF35H, is a single-pass type I membrane protein which belongs to the CD300 family. The CD300 family of myeloid immunoglobulin receptors includes activating ( CD300b, CD300e ) and inhibitory members ( CD300a, CD300f ), as well as molecules presenting a negative charge within their transmembrane domain ( CD300c, CD300d ). CD300A / IGSF12 is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes. It contains oneIg-like V-type ( immunoglobulin-like ) domain. CD300A / IGSF12 is an inhibitory receptor that may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. CD300c is a functional immune receptor able to deliver activating signals upon ligation in RBL-2H3 mast cells. CD300c signaling is partially mediated by a direct association with the immune receptor tyrosine-based activation motif-bearing adaptor FcεRγ. CD300a and CD300c play an important role in the cross-regulation of TNF-alpha and IFN-alpha secretion from plasmacytoid dendritic cells (pDCs). |
| Conjucates | APC |
| Others Formats | Unconjugated/FITC/PE |
| Antibody Types Available | 4 |
| Immunogen | Recombinant Human CD300A protein (TMPY-02013) |
| Antigen Species | Human |
| Biology Area | ITIM/ITAM Immunoreceptors and Related Molecules |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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