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Anti-CD3 epsilon/CD3e Antibody (8Z76)
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Catalog No. TMAC-00690
Alias TCRE, T3E, IMD18, CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e, CD3 ε, CD3 epsilon
Anti-CD3 epsilon/CD3e Antibody (8Z76) is a Rabbit antibody targeting CD3 epsilon/CD3e. Anti-CD3 epsilon/CD3e Antibody (8Z76) can be used in WB,IHC,IP,FCM.
Anti-CD3 epsilon/CD3e Antibody (8Z76)
Anti-CD3 epsilon/CD3e Antibody (8Z76)
😃Good
Catalog No. TMAC-00690Alias TCRE, T3E, IMD18, CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e, CD3 ε, CD3 epsilon
Anti-CD3 epsilon/CD3e Antibody (8Z76) is a Rabbit antibody targeting CD3 epsilon/CD3e. Anti-CD3 epsilon/CD3e Antibody (8Z76) can be used in WB,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-CD3 epsilon/CD3e Antibody (8Z76) is a Rabbit antibody targeting CD3 epsilon/CD3e. Anti-CD3 epsilon/CD3e Antibody (8Z76) can be used in WB,IHC,IP,FCM. |
| Synonyms | TCRE, T3E, IMD18, CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e, CD3 ε, CD3 epsilon |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of CD3 epsilon on different lysates using anti-CD3 epsilon antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: Human thymus. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin. 4. Flow cytometric analysis of Jurkat cells with CD3 epsilon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase. TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 23 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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