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Anti-CD3 epsilon/CD3e Antibody (2H914)
😃Good
Catalog No. TMAY-01399
Alias CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e
Anti-CD3 epsilon/CD3e Antibody (2H914) is a Rabbit antibody targeting CD3 epsilon/CD3e. Anti-CD3 epsilon/CD3e Antibody (2H914) can be used in WB,IP.
Anti-CD3 epsilon/CD3e Antibody (2H914)
Anti-CD3 epsilon/CD3e Antibody (2H914)
😃Good
Catalog No. TMAY-01399Alias CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e
Anti-CD3 epsilon/CD3e Antibody (2H914) is a Rabbit antibody targeting CD3 epsilon/CD3e. Anti-CD3 epsilon/CD3e Antibody (2H914) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, IP Reactivity: Human |
| Alias | CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e |
| Ig Type | Rabbit IgG |
| Clone | 2H914 |
| Reactivity | Human |
| Verified Activity | 1. CD3E was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg HuT78 Whole Cell Lysate. -2 µL anti-CD3E rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CD3E rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 23 kDa. -Observed band size: 23 kDa. 2. Anti-CD3E rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hu-T78 Whole Cell Lysate. -Lane B: Jurakt Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:23 kDa. -Observed band size:23 kDa(We are unsure as to the identity of these extra bands.) |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human CD3e / CD3 epsilon (rh CD3e / CD3 epsilon; TMPY-01191; NP_000724.1; Met1-Asp126). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human CD3e / CD3 epsilon Protein (TMPY-01191) |
| Antigen Species | Human |
| Biology Area | ITIM/ITAM Immunoreceptors and Related Molecules |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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