Anti-CD24 Polyclonal Antibody 3 is a Rabbit antibody targeting CD24. Anti-CD24 Polyclonal Antibody 3 can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat)

1. Blank control: SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-CD24 antibody (TMAB-03921)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Bone tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Mouse Cerebrum tissue lysates
Primary: Anti-CD24 (TMAB-03921) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 8 kD
Observed band size: 33 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: mouse CD24

Mouse

CD24

Signal transducer CD24

Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.