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Anti-CD16/FCGR3 Polyclonal Antibody
😃Good
Catalog No. TMAB-00337
Alias Fc receptor, IgG, low affinity III, CD16
Anti-CD16/FCGR3 Polyclonal Antibody is a Rabbit antibody targeting CD16/FCGR3. Anti-CD16/FCGR3 Polyclonal Antibody can be used in WB.
Anti-CD16/FCGR3 Polyclonal Antibody
Anti-CD16/FCGR3 Polyclonal Antibody
😃Good
Catalog No. TMAB-00337Alias Fc receptor, IgG, low affinity III, CD16
Anti-CD16/FCGR3 Polyclonal Antibody is a Rabbit antibody targeting CD16/FCGR3. Anti-CD16/FCGR3 Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CD16/FCGR3 Polyclonal Antibody is a Rabbit antibody targeting CD16/FCGR3. Anti-CD16/FCGR3 Polyclonal Antibody can be used in WB. |
| Synonyms | Fc receptor, IgG, low affinity III, CD16 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Raw264.7 (Mouse) Cell Lysate at 30 μg Primary: Anti-CD16 (TMAB-00337) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 50 kDa 2. Sample: Lane 1: Thymus (Rat) Lysate at 40 μg Lane 2: LympHnode (Mouse) Lysate at 40 μg Primary: Anti-CD16 (TMAB-00337) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 60 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane. Secreted. Exists also as a soluble receptor. |
| Tissue Specificity | Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020] |
| Immunogen | KLH conjugated synthetic peptide: human IGFR3/CD16 |
| Antigen Species | Human |
| Gene Name | FCGR3A |
| Gene ID | |
| Protein Name | Low affinity immunoglobulin gamma Fc region receptor III-A |
| Uniprot ID | |
| Biology Area | NK Cells,Human Lineage Negative,NK Cell Lineage,Monocytic Lineage,Neutrophil Lineage |
| Function | Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. |
| Molecular Weight | Theoretical: 27 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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