Anti-CD11b Antibody (3A561) is a Mouse antibody targeting CD11b. Anti-CD11b Antibody (3A561) can be used in FCM.

Mouse IgG1, k

3A561

Human

Blank control: granulocyte.
Primary Antibody (green line): Mouse Anti-CD11b antibody (TMAB-03826)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line) : Goat anti-mouse IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Monoclonal

Recombinant Protein: human CD11b

Human

ITGAM

Integrin alpha-M

Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.