Anti-BNIP3 Polyclonal Antibody is a Rabbit antibody targeting BNIP3. Anti-BNIP3 Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse)

Blank control: A43
Primary Antibody (green line): Rabbit Anti-BNIP3 antibody (TMAB-00246)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM

FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: human BNIP3

Human

Host Virus Interaction,Cancer,Mitochondrial markers,Hypoxia,Apoptosis,Mitochondrial,Bcl2 Family,Apoptosis,Mitochondrial,Metabolism,Response to hypoxia