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Anti-BCL2 Polyclonal Antibody 3
😃Good
Catalog No. TMAB-00198
Anti-BCL2 Polyclonal Antibody 3 is a Rabbit antibody targeting BCL2. Anti-BCL2 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
Anti-BCL2 Polyclonal Antibody 3
Anti-BCL2 Polyclonal Antibody 3
😃Good
Catalog No. TMAB-00198
Anti-BCL2 Polyclonal Antibody 3 is a Rabbit antibody targeting BCL2. Anti-BCL2 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | Sample: Lane 1: Spleen (Mouse) Lysate at 40 μg Lane 2: LympHnode (Mouse) Lysate at 40 μg Lane 3: LympHnode (Rat) Lysate at 40 μg Lane 4: MCF-7 (Human) Cell Lysate at 30 μg Lane 5: SiHa (Human) Cell Lysate at 30 μg Lane 6: Jurkat (Human) Cell Lysate at 30 μg Lane 7: A431 (Human) Cell Lysate at 30 μg Lane 8: Bone (Rat) Lysate at 40 μg Lane 9: Bone (Mouse) Lysate at 40 μg Lane 10: A549 (Human) Cell Lysate at 30 μg Lane 11: SH-SY5Y (Human) Cell Lysate at 30 μg Primary: Anti-Bcl-2 (TMAB-00198) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-300; ICC/IF: 1:100-500; IF: 1:200-1000; ELISA: 1:10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300. |
| Research Background | The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8). |
| Immunogen | KLH conjugated synthetic peptide: human Bcl-2 |
| Antigen Species | Human |
| Gene ID | 596 |
| Uniprot ID | |
| Biology Area | Cellular metabolic process,Response to hypoxia,Bcl 2 family,Metabolism,Bcl 2 family,Cell survival & death,Oncoproteins,Bcl2 Family,Apoptosis marker and proteins ELISA kits,Apoptosis,Hypoxia,Mitochondrial markers,Cancer,Mitochondrial |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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