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Anti-ATG1 Polyclonal Antibody
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Catalog No. TMAB-00162
Alias Unc-51-like kinase 1, Unc-51 like kinase 1(C. elegans), UNC51, Unc 51(C. elegans) like kinase 1, Unc 51 like kinase 1, UNC 51, ULK1, ULK 1, Serine/threonine protein kinase Unc51.1, Serine/threonine protein kinase ULK1, hATG1, Autophagy-related protein 1 homolog, ATG1A, ATG1, ATG 1
Anti-ATG1 Polyclonal Antibody is a Rabbit antibody targeting ATG1. Anti-ATG1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Anti-ATG1 Polyclonal Antibody
Anti-ATG1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00162Alias Unc-51-like kinase 1, Unc-51 like kinase 1(C. elegans), UNC51, Unc 51(C. elegans) like kinase 1, Unc 51 like kinase 1, UNC 51, ULK1, ULK 1, Serine/threonine protein kinase Unc51.1, Serine/threonine protein kinase ULK1, hATG1, Autophagy-related protein 1 homolog, ATG1A, ATG1, ATG 1
Anti-ATG1 Polyclonal Antibody is a Rabbit antibody targeting ATG1. Anti-ATG1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ATG1 Polyclonal Antibody is a Rabbit antibody targeting ATG1. Anti-ATG1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
| Alias | Unc-51-like kinase 1, Unc-51 like kinase 1(C. elegans), UNC51, Unc 51(C. elegans) like kinase 1, Unc 51 like kinase 1, UNC 51, ULK1, ULK 1, Serine/threonine protein kinase Unc51.1, Serine/threonine protein kinase ULK1, hATG1, Autophagy-related protein 1 homolog, ATG1A, ATG1, ATG 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Positive control: RSC96 Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody catalog number: TMAB-00162; Dilution: 0.5 μg in 100 μL 1X PBS containing 0.5% BSA 2. Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ATG1/ULK1 Polyclonal Antibody, Unconjugated (TMAB-00162) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: 293t (Human) Cell Lysate at 30 μg Primary: Anti-ATG1/ULK1 (TMAB-00162) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 116 kDa Observed band size: 116 kDa 4. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (ATG1/ULK1) polyclonal Antibody, Unconjugated (TMAB-00162) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 0.5μg /Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | ULK1 belongs to the serine/threonine protein kinase family. It is involved in axon growth and plays an essential role in neurite branching during sensory axon outgrowth. Knockdown of ULK1 results in impaired endocytosis of nerve growth factor (NGF), excessive axon arborization, and severely stunted axon elongation indicating that ULK1 mediates a non clathrin coated endocytosis in sensory growth cones. Knockdown of ULK1 also inhibits the autophagic response. It appears to act as a convergence point for multiple signals that regulate autophagy, and in turn interacts with a large number of autophagy related (Atg) proteins. |
| Immunogen | KLH conjugated synthetic peptide: human ATG1/ULK1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Other Kinases,Neurogenesis,Mitophagy fission and fusion,Autophagy |
| Molecular Weight | Theoretical: 116 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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