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Anti-ASCL2 Polyclonal Antibody

😃Good
Catalog No. TMAB-00151
Alias mASH-2, mASH2, ASH-2, Ascl2, Achaete-scute homolog 2

Anti-ASCL2 Polyclonal Antibody is a Rabbit antibody targeting ASCL2. Anti-ASCL2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-ASCL2 Polyclonal Antibody

Anti-ASCL2 Polyclonal Antibody

😃Good
Catalog No. TMAB-00151Alias mASH-2, mASH2, ASH-2, Ascl2, Achaete-scute homolog 2
Anti-ASCL2 Polyclonal Antibody is a Rabbit antibody targeting ASCL2. Anti-ASCL2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3737-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-ASCL2 Polyclonal Antibody is a Rabbit antibody targeting ASCL2. Anti-ASCL2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.
AliasmASH-2, mASH2, ASH-2, Ascl2, Achaete-scute homolog 2
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow)
Verified Activity
1. MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (ASCL2) polyclonal Antibody, Unconjugated (TMAB-00151) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ASCL2 Polyclonal Antibody, Unconjugated (TMAB-00151) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ASCL2 Polyclonal Antibody, Unconjugated (TMAB-00151) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ASCL2 Polyclonal Antibody, Unconjugated (TMAB-00151) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Blank control: K562. Primary Antibody (green line): Rabbit Anti-ASCL2 antibody (TMAB-00151)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 2ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundMembers of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes, both of which work together to activate DNA transcription. Class A proteins include the ubiquitously expressed E-box binding factors, namely E2A, ITF-2 and HEB, while class B proteins, such as MyoD, myogenin and Neuro D (BETA2), are transiently expressed and exhibit a much more limited tissue distribution. Working in opposition to these positively acting factors are a specialized group of basic helix-loop-helix (bHLH) transcription factors that function as dominant negative regulators and are involved in cell lineage determination and differentiation. ASCL2 is a 193 amino acid protein that localizes to the nucleus and contains one bHLH domain. Expressed in developing placental tissue, ASCL2 binds to DNA and functions as a transcriptional regulator that is involved in the maturation of neuronal precursors in the peripheral and central nervous systems.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: Human ASCL2
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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