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Anti-Argonaute-2 Polyclonal Antibody
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Catalog No. TMAB-00146
Anti-Argonaute-2 Polyclonal Antibody is a Rabbit antibody targeting Argonaute-2. Anti-Argonaute-2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Argonaute-2 Polyclonal Antibody
Anti-Argonaute-2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00146
Anti-Argonaute-2 Polyclonal Antibody is a Rabbit antibody targeting Argonaute-2. Anti-Argonaute-2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Sheep) |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AGO2) Polyclonal Antibody, Unconjugated (TMAB-00146) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Spleen (Mouse) Lysate at 40 μg Primary: Anti-eIF2C2 (TMAB-00146) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 97 kDa Observed band size: 97 kDa 3. Sample: Uterus (Mouse) Lysate at 40 μg Primary: Anti-eIF2C2 (TMAB-00146) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 97 kDa Observed band size: 97 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5). |
| Immunogen | KLH conjugated synthetic peptide: human AGO2 |
| Antigen Species | Human |
| Gene ID | 27161 |
| Uniprot ID | |
| Biology Area | RNAi,Nuclear Pore Complex,Argonaute and Piwi,Eukaryotic Initiation factors (eIF's) |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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