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Anti-APCDD1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00127
Alias HYPT1, HTS, HHS, FP7019, Drapc1, B7323, Apcdd1
Anti-APCDD1 Polyclonal Antibody is a Rabbit antibody targeting APCDD1. Anti-APCDD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-APCDD1 Polyclonal Antibody
Anti-APCDD1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00127Alias HYPT1, HTS, HHS, FP7019, Drapc1, B7323, Apcdd1
Anti-APCDD1 Polyclonal Antibody is a Rabbit antibody targeting APCDD1. Anti-APCDD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-APCDD1 Polyclonal Antibody is a Rabbit antibody targeting APCDD1. Anti-APCDD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | HYPT1, HTS, HHS, FP7019, Drapc1, B7323, Apcdd1 |
| Reactivity | Human (predicted:Mouse,Rat,Chicken,Dog,Rabbit) |
| Verified Activity | 1. Formalin-fixed and paraffin embedded human stomach labeled with Rabbit Anti-APCDD1 Polyclonal Antibody (TMAB-00127) at 1:250 room temperature overnight followed by conjugation to secondary antibody. 2. Sample: DU145 Cell (Human) Lysate at 40 μg Primary: Anti-APCDD1 (TMAB-00127) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (APCDD1) Polyclonal Antibody, Unconjugated (TMAB-00127) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | APCDD1 is a novel protein that has been shown to be a target of Wnt/beta catenin signaling pathway in cancer cell lines. APCDD1 is overexpressed in colorectal carcinogenesis and is deregulated in CTNNB1 mutated Wilms tumors. [FUNCTION] Probably plays a role in colorectal tumorigenesis. May be a developmental target gene of the Wnt/Beta-catenin pathway. [SUBCELLULAR LOCATION] Membrane; Single-pass type I membrane protein (Potential). [TISSUE SPECIFICITY] Abundantly expressed in heart, pancreas, prostate and ovary. Moderately expressed in lung, liver, kidney, spleen, thymus, colon and peripheral lymphocytes. [INDUCTION] Transcriptionally regulated by the CTNNB1/TF7L2complex. |
| Immunogen | KLH conjugated synthetic peptide: human APCDD1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Signal transduction,Surface Molecules |
| Molecular Weight | Theoretical: 56 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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