Anti-APCDD1 Polyclonal Antibody is a Rabbit antibody targeting APCDD1. Anti-APCDD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Human (predicted:Mouse,Rat,Chicken,Dog,Rabbit)

1. Formalin-fixed and paraffin embedded human stomach labeled with Rabbit Anti-APCDD1 Polyclonal Antibody (TMAB-00127) at 1:250 room temperature overnight followed by conjugation to secondary antibody.
2. Sample: DU145 Cell (Human) Lysate at 40 μg
Primary: Anti-APCDD1 (TMAB-00127) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (APCDD1) Polyclonal Antibody, Unconjugated (TMAB-00127) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human APCDD1

Human

APCDD1

Protein APCDD1

Signal transduction,Surface Molecules

Negative regulator of the Wnt signaling pathway. Inhibits Wnt signaling in a cell-autonomous manner and functions upstream of beta-catenin. May act via its interaction with Wnt and LRP proteins. May play a role in colorectal tumorigenesis.