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Anti-APCDD1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00127
Alias HYPT1, HTS, HHS, FP7019, Drapc1, B7323, Apcdd1

Anti-APCDD1 Polyclonal Antibody is a Rabbit antibody targeting APCDD1. Anti-APCDD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-APCDD1 Polyclonal Antibody

Anti-APCDD1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00127Alias HYPT1, HTS, HHS, FP7019, Drapc1, B7323, Apcdd1
Anti-APCDD1 Polyclonal Antibody is a Rabbit antibody targeting APCDD1. Anti-APCDD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-APCDD1 Polyclonal Antibody is a Rabbit antibody targeting APCDD1. Anti-APCDD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasHYPT1, HTS, HHS, FP7019, Drapc1, B7323, Apcdd1
Reactivity
Human (predicted:Mouse,Rat,Chicken,Dog,Rabbit)
Verified Activity
1. Formalin-fixed and paraffin embedded human stomach labeled with Rabbit Anti-APCDD1 Polyclonal Antibody (TMAB-00127) at 1:250 room temperature overnight followed by conjugation to secondary antibody.
2. Sample: DU145 Cell (Human) Lysate at 40 μg
Primary: Anti-APCDD1 (TMAB-00127) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (APCDD1) Polyclonal Antibody, Unconjugated (TMAB-00127) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type I membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundAPCDD1 is a novel protein that has been shown to be a target of Wnt/beta catenin signaling pathway in cancer cell lines. APCDD1 is overexpressed in colorectal carcinogenesis and is deregulated in CTNNB1 mutated Wilms tumors. [FUNCTION] Probably plays a role in colorectal tumorigenesis. May be a developmental target gene of the Wnt/Beta-catenin pathway. [SUBCELLULAR LOCATION] Membrane; Single-pass type I membrane protein (Potential). [TISSUE SPECIFICITY] Abundantly expressed in heart, pancreas, prostate and ovary. Moderately expressed in lung, liver, kidney, spleen, thymus, colon and peripheral lymphocytes. [INDUCTION] Transcriptionally regulated by the CTNNB1/TF7L2complex.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human APCDD1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Signal transduction,Surface Molecules
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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