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Anti-Alkaline Phosphatase (Placental type) /ALPP Antibody (8Z127) is a Rabbit antibody targeting Alkaline Phosphatase (Placental type) /ALPP. Anti-Alkaline Phosphatase (Placental type) /ALPP Antibody (8Z127) can be used in WB,ICC/IF,IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $496 | 7-10 days |
Description | Anti-Alkaline Phosphatase (Placental type) /ALPP Antibody (8Z127) is a Rabbit antibody targeting Alkaline Phosphatase (Placental type) /ALPP. Anti-Alkaline Phosphatase (Placental type) /ALPP Antibody (8Z127) can be used in WB,ICC/IF,IHC. |
Synonyms | placental type, Placental alkaline phosphatase 1, ALPP and PLAP, Alkaline phosphatase Regan isozyme, Alkaline Phosphatase |
Ig Type | IgG |
Reactivity | Human |
Verified Activity | 1. Western blot analysis of PLAP on human placenta tissue (1) and Hela cell (2) lysate using anti-PLAP antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PLAP antibody. Counter stained with hematoxylin. 3. ICC staining PLAP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining PLAP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Alkaline phosphatases (AP) are glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+-metallated glycoproteins that catalyze the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. Placental alkaline phosphatase (also known as PLAP, ALPP, PALP, placental ALP-1 or Regan isozyme) is a 530 amino acid, tissue-specific AP that is expressed in the placenta, the serum of pregnant women and ectopically expressed in various cancers, including those of the ovary and testis. PLAP may assist in guiding migratory cells and transporting specific molecules, such as fatty acids and immunoglobulins, across the plasma membrane. The three tissue-specific APs identified in human, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous and their genes are clustered on chromosome 2q. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 70 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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