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Anti-AKT1/2/3 Antibody (8U152)
😃Good
Catalog No. TMAC-00126
Alias STK-2, RAC-PK-gamma, RAC-PK-beta, RAC-PK-alpha, RAC-gamma serine/threonine-protein kinase, RAC-beta serine/threonine-protein kinase, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, Protein kinase B gamma, Protein kinase B beta, Protein kinase B alpha, Protein kinase B, Protein kinase Akt-3, Protein kinase Akt-2, PKBG, PKB RAC, PKB gamma, PKB beta, PKB alpha, PKB, EC 2.7.11.1, AKT3, AKT2, AKT1, AKT 3, AKT 2, AKT 1
Anti-AKT1/2/3 Antibody (8U152) is a Rabbit antibody targeting AKT1/2/3. Anti-AKT1/2/3 Antibody (8U152) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-AKT1/2/3 Antibody (8U152)
Anti-AKT1/2/3 Antibody (8U152)
😃Good
Catalog No. TMAC-00126Alias STK-2, RAC-PK-gamma, RAC-PK-beta, RAC-PK-alpha, RAC-gamma serine/threonine-protein kinase, RAC-beta serine/threonine-protein kinase, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, Protein kinase B gamma, Protein kinase B beta, Protein kinase B alpha, Protein kinase B, Protein kinase Akt-3, Protein kinase Akt-2, PKBG, PKB RAC, PKB gamma, PKB beta, PKB alpha, PKB, EC 2.7.11.1, AKT3, AKT2, AKT1, AKT 3, AKT 2, AKT 1
Anti-AKT1/2/3 Antibody (8U152) is a Rabbit antibody targeting AKT1/2/3. Anti-AKT1/2/3 Antibody (8U152) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-AKT1/2/3 Antibody (8U152) is a Rabbit antibody targeting AKT1/2/3. Anti-AKT1/2/3 Antibody (8U152) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Alias | STK-2, RAC-PK-gamma, RAC-PK-beta, RAC-PK-alpha, RAC-gamma serine/threonine-protein kinase, RAC-beta serine/threonine-protein kinase, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, Protein kinase B gamma, Protein kinase B beta, Protein kinase B alpha, Protein kinase B, Protein kinase Akt-3, Protein kinase Akt-2, PKBG, PKB RAC, PKB gamma, PKB beta, PKB alpha, PKB, EC 2.7.11.1, AKT3, AKT2, AKT1, AKT 3, AKT 2, AKT 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of AKT1/2/3 on MCF-7 cell lysates using anti-AKT1/2/3 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin. 6. ICC staining AKT1/2/3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining AKT1/2/3 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining AKT1/2/3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of A549 cells with AKT1/2/3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 56 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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